About the Proteomics Team
The Proteomics Team utilizes cutting-edge technologies to produce detailed proteomic profiles of infected red blood cells (RBCs) and/or their membrane fractions, and quantitative comparisons of infected RBCs and bone marrow aspirates in selected experiments, from non-human primates infected with Plasmodium pathogens. The results are provided in the form of: a) positive identifications of the host and parasite proteins and post-translational modifications (PTM) obtained by in-depth screening of peptide fractions generated by digestion or chemical cleavage of the proteomes; and b) quantitative comparisons of relevant samples by stable isotope labeling/tandem mass spectrometry (MS/MS) including the iTRAQ and ICAT technologies and stable isotope dilution mass spectrometry approach (MRM-MS/MS). The data is presented in the form of summary tables listing identified and functionally annotated proteins and their sites of PTM, as well as relative quantitative ratios obtained for individual proteins, allowing comparisons of experimentally related samples.
Jortzik, E., Kehr, S., Becker, K. (2011). "Post-translational Modifications in Apicomplexan Parasites." Progress in Parasitology 2: 93-120.
Pasini, E.M., Lutz, H.U., Mann, M., Thomas, A.W. (2010). "Red Blood Cell (RBC) membrane proteomics -- Part II: Comparative proteomics and RBC patho-physiology." Journal of Proteomics, 73(3): 421-435. View in PubMed
Picotti, P., Rinner, O., Stallmach, R., Dautel, F., Farrah, T., Domon, B., Wenschuh, H., Aebersold, R. (2010). "High-throughput generation of selected reaction-monitoring assays for proteins and proteomes." Nature Methods, 7(1): 43-46. View in PubMed
Shadforth, I.P., Dunkley, T.P.J., Lilley, K.S., Bessand, C. (2005). "i-Tracker: For quantitative proteomics using iTRAQ™." BMC Genomics, 6: 145-151. View in PubMed